Midori Green Advance
1 ml MIDORIGreen Advance DNA/RNA stain for in gel or poststaining
1 812 Kč bez DPH
MIDORIGreen Advance is a safe alternative to the traditional nucleic acid stain ethidium bromide. It is a non-carcinogenic and less mutagenic dye for detecting dsDNA, ssDNA and RNA in agarose gels with a very high sensitivity. MIDORIGreen Advance can utilize with UV light or with our innovative Blue/Green LED technology.
- Perfect in-gel staining of DNA/RNA in agarose gels
- No toxicity, non-carcinogenic
- Safe alternative of Ethidium bromide
- High fluorescence
- Optimal for Blue/Green LED Technology
The next generation of in-gel staining
MIDORIGreen Advance is a second-generation non-carcinogenic dye. Optimised for a brighter signal when excited by UV-light or blue/green light. It maintained the advantages, such as being non-carcinogenic and having an excellent noise-to-signal ratio. As seen in Fig. 1 MIDORIGreen Advance is even better than Ethidium bromide when using the Blue/Green LED technology.
Fig. 1: Comparison of sensitivity between MIDORIGreen Advance (left green) and Ethidium Bromide (right red) using a Blue/Green LED Transilluminator.
MIDORIGreen Advance shows a very high sensitivity even for small DNA fragments. The dilution factor of MIDORIGreen Advance can be as high as 1:25000. Hence, 4-6 μl are enough for the staining of 100 ml agarose gel, resulting in ~17 to 25 liters of stained agarose gels.
It is essential for a good replacement of the mutagenic DNA stain Ethidium Bromide to deliver strong signals. MIDORIGreen Advance delivers signals with a comparable intensity. Nonetheless, the safety of the user must not be compromised. Hence, several tests were performed with MIDORIGreen Advance and according to those tests, MIDORIGreen Advance is safe.