Midori Green Advance

MIDORIGreen Advance

MIDORIGreen Advance is a safe alternative to the traditional nucleic acid stain ethidium bromide. It is a non-carcinogenic and less mutagenic dye for detecting dsDNA, ssDNA and RNA in agarose gels with a very high sensitivity. MIDORIGreen Advance can utilize with UV light or with our innovative Blue/Green LED technology.

• Perfect in-gel staining of DNA/RNA in agarose gels
• No toxicity, non-carcinogenic
• Safe alternative of Ethidium bromide
• High fluorescence
• Optimal for Blue/Green LED Technology

The next generation of in-gel staining

MIDORIGreen Advance is a second-generation non-carcinogenic dye. Optimised for a brighter signal when excited by UV-light or blue/green light. It maintained the advantages, such as being non-carcinogenic and having an excellent noise-to-signal ratio. As seen in Fig. 1 MIDORIGreen Advance is even better than Ethidium bromide when using the Blue/Green LED technology.

Fig. 1: Comparison of sensitivity between MIDORIGreen Advance (left green) and Ethidium Bromide (right red) using a Blue/Green LED Transilluminator.

MIDORIGreen Advance shows a very high sensitivity even for small DNA fragments. The dilution factor of MIDORIGreen Advance can be as high as 1:25000. Hence, 4-6 μl are enough for the staining of 100 ml agarose gel, resulting in ~17 to 25 liters of stained agarose gels.

Proven safety

It is essential for a good replacement of the mutagenic DNA stain Ethidium Bromide to deliver strong signals. MIDORIGreen Advance delivers signals with a comparable intensity. Nonetheless, the safety of the user must not be compromised. Hence, several tests were performed with MIDORIGreen Advance and according to those tests, MIDORIGreen Advance is safe.

 Download here the MIDORIGreen Advance safety report.