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
FastGene® Western ECL Kit je vylepšený chemiluminiscenční (ECL) substrát na bázi luminolu.
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The FastGene® Western ECL Kit is an enhanced chemiluminescent (ECL) substrate based on luminol.
It is used to detect horseradish peroxidase (HRP) – conjugated secondary antibodies.
The high femtogram or low picogram detection of antigen is enabled by FastGene® Western ECL Kit brilliant sensitivity and long signal duration.
This long chemiluminescent signal duration makes it possible to detect signals on both digital and film-based imaging systems without any loss in signal intensity.
Appropriate primary and secondary antibody dilutions are suggested for attaining optimal signal intensity and duration.
Mix the luminol solution and peroxide solution in a 1:1 ratio, and thoroughly agitate the chemiluminescent substrate solution well for preparing the 0.1 ml of solution / cm2 of membrane.
Place the membrane with the protein side up and remove the membrane from the chemiluminescent substrate solution. Image the membrane with a digital imager.