qPCRBIO SyGreen 1-Step Go Lo-ROX (100 reakcí)
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  • qPCRBIO SyGreen 1-Step Go Lo-ROX (100 reakcí)

qPCRBIO SyGreen 1-Step Go Lo-ROX (100 reakcí)

PB25.31-01

qPCRBIO SyGreen 1-Step Kit byl navržen pro rychlou, vysoce specifickou a ultracitlivou syntézu cDNA a PCR v reálném čase v jedné zkumavce.

3 332 Kč Vč. DPH

2 754 Kč bez DPH

qPCRBIO SyGreen 1-Step Kits byly navrženy pro rychlou, vysoce specifickou a ultracitlivou syntézu cDNA a PCR v reálném čase v jedné zkumavce.

Vyberte si z qPCRBIO SyGreen 1-Step Detect pro detekci cílů s extrémně nízkým počtem kopií nebo qPCRBIO SyGreen 1-Step Go pro rychlé a přesné výsledky z vysokých koncentrací RNA templátu.


    Thermostable reverse transcriptase 45°C to 55°C 

  • Advanced RNase inhibitor 
  • Non-PCR inhibiting intercalating dye 
  • Rapid extension rate for early Ct values 
  • Market-leading sensitivity 
  • Increased limit of detection 
  • Antibody-mediated hot start PCR 
  • Compatible on all standard and fast cycling real-time PCR platforms 

qPCRBIO SyGreen 1-Step Kits can be used to quantify any RNA template including mRNA, total RNA and viral RNA sequences. qPCRBIO SyGreen 1-Step Detect is designed for sensitivity and is ideally suited to the detection of extremely low copy number targets. qPCRBIO SyGreen 1-Step Go gives the earliest Ct and is formulated for rapid and accurate results from high template concentrations.

The kits include a thermostable and extremely active modified MMLV reverse transcriptase and advanced RNase inhibitor that prevents degradation of RNA by contaminating RNase. PCR amplification is powered by antibody-mediated hot start technology, preventing the formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity. Combining the latest developments in polymerase technology and advanced buffer chemistry we offer market- leading performance with minimal or no optimisation.

Aplikace:

  • Absolute quantification
  • Relative gene expression analysis
  • Detection of extremely low copy number targets
  • qPCRBIO SyGreen 1-Step Detect recommended for template amounts of 1pg - 10ng total RNA or >0.01pg mRNA per reaction
  • qPCRBIO SyGreen 1-Step Go recommended for template amounts of 10pg - 100ng total RNA or >0.01pg mRNA per reaction