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qPCR SyGreen 1-Step Hi-ROX (300x20ul reakcí)
PB25.12-03
qPCRBIO SyGreen 1-Step Kit byl navržen pro rychlou, vysoce specifickou a ultracitlivou syntézu cDNA a PCR v reálném čase v jedné zkumavce.
8 821 Kč
Vč. DPH
7 290 Kč bez DPH
qPCRBIO SyGreen 1-Step Kits byly navrženy pro rychlou, vysoce specifickou a ultracitlivou syntézu cDNA a PCR v reálném čase v jedné zkumavce.
Vyberte si z qPCRBIO SyGreen 1-Step Detect pro detekci cílů s extrémně nízkým počtem kopií nebo qPCRBIO SyGreen 1-Step Go pro rychlé a přesné výsledky z vysokých koncentrací RNA templátu.
Thermostable reverse transcriptase 45°C to 55°C
- Advanced RNase inhibitor
- Non-PCR inhibiting intercalating dye
- Rapid extension rate for early Ct values
- Market-leading sensitivity
- Increased limit of detection
- Antibody-mediated hot start PCR
- Compatible on all standard and fast cycling real-time PCR platforms
qPCRBIO SyGreen 1-Step Kits can be used to quantify any RNA template including mRNA, total RNA and viral RNA sequences. qPCRBIO SyGreen 1-Step Detect is designed for sensitivity and is ideally suited to the detection of extremely low copy number targets. qPCRBIO SyGreen 1-Step Go gives the earliest Ct and is formulated for rapid and accurate results from high template concentrations.
The kits include a thermostable and extremely active modified MMLV reverse transcriptase and advanced RNase inhibitor that prevents degradation of RNA by contaminating RNase. PCR amplification is powered by antibody-mediated hot start technology, preventing the formation of primer dimers and non-specific products leading to improved reaction sensitivity and specificity. Combining the latest developments in polymerase technology and advanced buffer chemistry we offer market- leading performance with minimal or no optimisation.
Aplikace:
- Absolute quantification
- Relative gene expression analysis
- Detection of extremely low copy number targets
- qPCRBIO SyGreen 1-Step Detect recommended for template amounts of 1pg - 10ng total RNA or >0.01pg mRNA per reaction
- qPCRBIO SyGreen 1-Step Go recommended for template amounts of 10pg - 100ng total RNA or >0.01pg mRNA per reaction
qPCRBIO SyGreen 1-Step Kit byl navržen pro rychlou, vysoce specifickou a ultracitlivou syntézu cDNA a PCR v reálném čase v jedné zkumavce.