PCRBIO Ultra Mix (400 reactions)

• Increased PCR success rates with difficult templates
• Exhibits high tolerance to PCR inhibitors
• Efficient specific amplification from complex templates including GC-rich sequences (up to 80%)
• Antibody-mediated hot start for unrivalled detection of low copy number templates
• Advanced buffer chemistry including Mg and dNTPs
• High yields under standard and fast PCR conditions
• 3 fold higher fidelity than Taq
• Available as a convenient 2x ready mix with the option of a red dye for direct gel loading

PCRBIO Ultra Polymerase is a highly robust enzyme, designed for efficient and reliable amplification of challenging and complex targets, even under difficult conditions such as the presence of inhibitors. The enzyme and buffer system have been developed to give superior PCR performance and higher success rates on a broad range of templates, including complex genomic DNA and targets with a high GC content (up to 80% GC).

PCRBIO Ultra Polymerase exhibits a high tolerance to PCR inhibitors making it the ideal choice for colony and crude sample PCR. PCR products generated are A-tailed and may be cloned into TA cloning vectors. The enzyme is supplied with an optimised buffer containing dNTPs and MgCl2.

Our antibody-mediated hot start formulation prevents the formation of primer dimers and non-specific products, enabling specific and sensitive amplification from low copy number target sequences. For added convenience PCRBIO Ultra Polymerase is also available as a 2x ready mix containing all reaction components except primers and template. PCRBIO Ultra Mix Red contains a preloaded red dye suitable for direct loading and tracking during agarose gel electrophoresis.

Applications

• Difficult PCR - GC and AT-rich DNA
• Low copy template detection
• Crude sample PCR
• Colony PCR
• Long range PCR
• Multiplex PCR
• TA cloning
• Next generation sequencing