0
VeriFi™ Polymerase (500 units)
PB10.42-05
VeriFi™ Polymerase je všestranná a robustní DNA polymeráza s vysokou přesností navržená pro všechny aplikace PCR, kde je vyžadována větší přesnost sekvence.
14 157 Kč
Vč. DPH
11 700 Kč bez DPH
Improved DNA binding and increased processivity result in shorter extension times, higher yields and the ability to amplify longer and more difficult targets.
VeriFi™ Polymerase was formerly known as PCRBIO VeriFi™ Polymerase
Features
- High temperature cycling – up to 100 °C denaturation to better separate GC-rich templates
- Increased PCR success rates with complex genomic templates (17.5 kb and over)
- High yields under standard and fast PCR conditions (10-30 s/kb)
- Efficient and specific amplification from challenging templates including GC and AT-rich sequences
- 100x higher fidelity than Taq DNA polymerase
- Generates blunt-end PCR products
- Available as a convenient 2x ready mix with the option of a red dye for direct gel loading
VeriFi™ Polymerase is derived from Pfu DNA polymerase for its 3’-5’ exonuclease (proofreading) activity. The enzyme is engineered with proprietary mutations that significantly increase processivity, resulting in shorter extension times (10-30 s/kb), higher yields and the ability to amplify longer and more difficult targets, including eukaryotic genomic templates in excess of 17.5 kb.
The high accuracy and enhanced 3’-5’ exonuclease activity of VeriFi™ Polymerase result in fidelity that is approximately 100 times higher than Taq DNA polymerase. The enzyme is ideally suited to applications where greater accuracy is required, such as cloning, site-directed mutagenesis and sequencing. PCR products generated with this range of products are blunt ended.
VeriFi™ Polymerase enables high fidelity PCR of a wide range of targets and fragment sizes with minimal or no optimisation required. The enzyme is provided with an advanced buffer system including both dNTPs and Mg. For the most difficult templates, such as highly GC-rich sequences or those with complex secondary structures, users have the option of adding the VeriMax Enhancer to the reaction mix for improved PCR performance.
VeriFi™ Polymerase is also available as a convenient 2x ready mix containing all reaction components apart from primers and template. Our 2x red mix contains a red dye suitable for direct loading and tracking during agarose gel electrophoresis.
Applications
- High fidelity PCR
- Long range PCR
- Site-directed mutagenesis
- Cloning
- Sequencing
VeriFi -varianty:
PB10.42-01 VeriFi Polymerase,100 Units [1 x 0.05mL 2u/µL] & [1 x 1.7mL buffer]
PB10.42-05 VeriFi Polymerase, 500 Units [1 x 0.250mL 2u/µL] & [3 x 1.7mL buffer]
PB10.43-01 VeriFi Mix 100 x 50µL Reactions 2 x 1.25mL
PB10.43-05 VeriFi Mix 500 x 50µL Reactions 2 x (5 x 1.25mL)
PB10.44-01 VeriFi Mix Red 100 x 50µL Reactions 2 x 1.25mL
PB10.44-05 VeriFi Mix Red 500 x 50µL Reactions 2 x (5 x 1.25mL)
VeriFi™ Polymerase je všestranná a robustní DNA polymeráza s vysokou přesností navržená pro všechny aplikace PCR, kde je vyžadována větší přesnost sekvence.