PCR Cycler Check™ OneStep, 100 reactions

False negative PCR results are highly critical and might be due to a defective PCR cycler. Verification of the correct temperature control of the equipment in-use is generally a strenuous task. Furthermore, compliance of PCR cyclers with quality management systems is not easy to achieve.
The PCR Cycler Check™ Kits provide temperature sensitive PCR reactions to monitor an upper and lower temperature range in one run. The primer/ probe sequences were designed to be extremely sensitive to fluctuations in temperature and homogeneity, precision of the temperature control and timing.
Amplification will be altered when temperature deviates by more than 2 °C from the set value. Cycler performance is tested with typical PCR settings to reflect most common applications. As an additional indicator for accurate temperature control of the cycler, the included pre-adjusted target concentrations are only amplified by highly efficient PCR reactions.

Cycler Types

Conventional thermocyclers for endpoint PCR

Benefits

• No extra equipment, instrument or software needed.
• Includes all you need: Lyophilized primers, polymerase, nucleotides, rehydration buffer, gel loading buffer with front marker, and marker.

Recommended Use

Applicable to any block PCR cycler in research or industrial quality control to test the legal requirements for testing the reliability of instruments used for analysis (ISO 17025, EN 45001, ISO 13485, GLP, GMP).

Kit Components

Lyophilized primer sets, polymerase and nucleotides
Rehydration buffer
Size marker for optimized gel evaluation
Gel loading buffer with front marker (only for Cat. No. 57-2103)

Package Sizes

• Cat. No. 57-2102PCR Cycler Check™ Advance 6 strips, 8 vials each
• Cat. No. 57-2103PCR Cycler Check™ OneStep 100 reactions

Result Evaluation

Gel electrophoresis for endpoint PCR

Required Consumables

Reagents for agarose gel electrophoresis
For Cat. No. 57-2103 only: PCR reaction tubes

Required Lab Devices

PCR cycler
Microcentrifuge
Pipettes
Agarose gel electrophoresis and documentation system

Shelf Life and Storage

Store the unopened components at +2 °C to +8 °C until the expiry date indicated on the label. All the reagents must be used directly after rehydration. For Cat. No. 57-2103: the convenient format of the Validation Reactions in single-use aliquots (1 validation assay per vial) prevents repeated freeze-thawing and waste of valuable reagents.