IsoFast™ Bst Polymerase
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  •  IsoFast™ Bst Polymerase

IsoFast™ Bst Mix

PB80.12-01

100 x 25μL Reactions

Applications

  • Whole genome amplification
  • Multiple displacement amplification
  • Isothermal amplification
  • Loop mediated isothermal amplification (LAMP)
  • Molecular diagnostics
  • Field diagnostics
6 346 Kč Vč. DPH

5 245 Kč bez DPH

IsoFast™ Bst Polymerase is a recombinant form of the large fragment of Bst DNA polymerase, containing 5′

-3′ polymerase activity and strand displacement activity.

The enzyme offers fast amplification and strong strand displacement capabilities, making it ideal for nucleic acid amplification methods such as whole genome amplification, multiple displacement amplification and isothermal amplification.

Features

  • Has strand-displacing 5′-3′ polymerase activity
  • Lacks 5′-3′ exonuclease activity
  • DNA synthesis is performed at a constant temperature
  • Operates over a broad temperature range, with an optimum of 65ºC
  • Gives rapid and consistent amplification across a wide range of templates
  • Supplied with an advanced 2-part buffer system for higher yields under difficult conditions
  • 30 minute protocol
  • Flexible formats with and without fluorescent dye
  • Also available as a 2x mix and as a dual enzyme 1-step system for RNA amplification
  • Glycerol-free enzyme

Varianty:

kat.č. Název Balení
PB80.10-01 IsoFast Bst Polymerase 1600 units
PB80.10-08 IsoFast Bst Polymerase 8000 units
PB80.11-01 IsoFast Bst Polymerase with Dye 1600 units
PB80.11-08 IsoFast Bst Polymerase with Dye 8000 units
     
PB80.12-01 IsoFast Bst Mix 100 reactions
PB80.12-05 IsoFast Bst Mix 500 reactions
     
PB80.21-01 IsoFast Bst 1-Step Mix 100 reactions
PB80.21-05 IsoFast Bst 1-Step Mix 500 reactions
     
PB80.30-02 Fluorescent Dye  200 reactions
PB80.30-10 Fluorescent Dye  1000 reactions