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BlueStar Plus Prestained Protein Marker (500 µl)
MWP04
1 x 500 µl Protein Marker in loading buffer, sufficient for 100 mini gels or 50 large gels
Broad range: 10 – 240 kDa
3 202 Kč
Vč. DPH
2 646 Kč bez DPH
BlueStar PLUS Prestained Protein Marker
The BlueStar PLUS Prestained Protein Marker is a three color protein standard with 12 prestained proteins covering a wide range molecular weights from 10 – 240
kDa. The Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins.
- Broad range: 10 – 240 kDa
- High performance: 12 bands with 3 colours (blue, red and green)
- Ready to use: Supplied in a loading buffer for direct loading on gels
- Easy to identify: The 25 (green) and 75 kDa (red) are reference bands
- Sharp bands
Contents
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, 2 % SDS, 0,2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).
Storage
Store at -20°C for 24 months.
Stable at +4°C for 3 months.
Stable at +25°C for 2 weeks.
Recommendations for Loading
- Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
- Mix thoroughly, to ensure that the solution is homogeneous.
- Load the following volumes of the ladder on SDS-polyacrylamide gel:
– 5 μl per well for mini-gels, 3 μl per well for blots;
– 10 μl per well for large gels, 6 μl per well for blots
1 x 500 µl Protein Marker in loading buffer, sufficient for 100 mini gels or 50 large gels
Broad range: 10 – 240 kDa