BlueStar Plus Prestained Protein Marker (500 µl)
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  • BlueStar Plus Prestained Protein Marker (500 µl)

BlueStar Plus Prestained Protein Marker (500 µl)

MWP04

1 x 500 µl Protein Marker in loading buffer, sufficient for 100 mini gels or 50 large gels

Broad range:  10 – 240 kDa

3 202 Kč Vč. DPH

2 646 Kč bez DPH

BlueStar PLUS Prestained Protein Marker

The BlueStar PLUS Prestained Protein Marker is a three color protein standard with 12 prestained proteins covering a wide range molecular weights from 10 – 240
kDa. The Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximate sizing of proteins.

Lauferhalten dse BlueStar PLUS Proteinmarkers in Tris-Glycin, Bis-Tris MOPS und Bis Tris MES Puffer

 

  • Broad range: 10 – 240 kDa
  • High performance: 12 bands with 3 colours (blue, red and green)
  • Ready to use: Supplied in a loading buffer for direct loading on gels
  • Easy to identify: The 25 (green) and 75 kDa (red) are reference bands
  • Sharp bands

 

Contents

Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, 2 % SDS, 0,2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol).

 

Storage

Store at -20°C for 24 months.
Stable at +4°C for 3 months.
Stable at +25°C for 2 weeks.

 

Recommendations for Loading

  1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
  2. Mix thoroughly, to ensure that the solution is homogeneous.
  3. Load the following volumes of the ladder on SDS-polyacrylamide gel:
    – 5 μl per well for mini-gels, 3 μl per well for blots;
    – 10 μl per well for large gels, 6 μl per well for blots