Pink Prestained Protein Marker (500 µl)

PiNK Prestained Protein Ladder

The PiNK Prestained Protein Ladder contains 11 proteins (8 pink and 3 blue bands) that resolve into sharp, tight bands in the range of 5-175 kDa. The PiNK Prestained Protein Ladder allows you to monitor molecular weight separation during electrophoresis, estimate molecular weights of proteins of interest, and evaluate western transfer efficiency.

• Broad range: 5 – 175 kDa
• High performance: 11 bands with 2 colours (pink and blue)
• Ready to use: Supplied in a loading buffer for direct loading on gels.
• Sharp bands

Quality Control

Tested in SDS-polyacrylamide gel electrophoresis and Western blotting.

Storage Buffer

20 mM Tris-phosphate pH 7.5 (at 25°C ), 2 % SDS, 1 mM
Dithiothreitol, 4.8 M Urea and 12 % (v/v) Glycerol.
(Approximately 0.2 ~ 0.4 mg/ml of each protein in the buffer)

Storage

Store at -20 °C for 12 months.
Stable at +4 °C for 3 months.

Recommendations for Loading

1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
2. Mix thoroughly to ensure the solution is homogeneous.
3. Load the following volumes of the ladder on SDS-polyacrylamide gel:

– 5 μl per well for mini-gels, 2.5 μl per well for blots
– 10 μl per well for large gels, 5 μl per well for blots