BlueStar Prestained Protein Marker (500 µl)
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  • BlueStar Prestained Protein Marker (500 µl)

BlueStar Prestained Protein Marker (500 µl)

MWP03

1 x 500 µl Protein Marker in loading buffer, sufficient for 100 mini gels or 50 large gels

Broad range: 10 – 180 kDa

2 998 Kč Vč. DPH

2 478 Kč bez DPH

BlueStar Prestained Protein Marker

The BlueStar Prestained Protein Ladder is a three color protein standard with 10 prestained proteins covering a wide range molecular weights from 10 to 180 kDa. The BlueStar Prestained Protein Ladder is designed for monitoring protein separation during SDS-polyacrylamide gel electrophoresis, verification of Western transfer efficiency on membranes (PVDF, nylon or nitrocellulose) and for approximating the size of proteins.

 

Lauferhalten dse BlueStar Proteinmarkers in Tris-Glycin, Bis-Tris MOPS und Bis Tris MES Puffer

 

  • Broad range: 10 – 180 kDa
  • High performance: 10 bands with 3 colours (blue, orange and green)
  • Ready to use: Supplied in a loading buffer for direct loading on gels.
  • Reference bands: 28 kDa (green) and 75 kDa (orange)
  • Sharp bands

 

Storage Buffer

20 mM Disodium hydrogen phosphate pH 8.0 (at 25°C ), 2 % SDS, 0.2% Triton X-100,10 mM 2-Mercaptoethanol, 6 M Urea and 27% (v/v) Glycerol.

 

Storage

Stable at -20°C for 12 months.
Stable at +4°C for 3 months.

 

Recommendations for Loading

1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
2. Mix thoroughly, to ensure that the solution is homogeneous.
3. Load the following volumes of the ladder on SDS-polyacrylamide gel:

  • 5 μl per well for mini-gels, 3 μl per well for blots
  • 10 μl per well for large gels, 6 μl per well for blots