IPTG

Product Overview:

IPTG (Isopropyl B-D-thiogalactopyranoside), Dioxane-Free is an analogue of allolactose that binds specifically to the repressor protein of the lac operon and induces expression of β-galactosidase in Escherichia coli. Unlike allolactose, IPTG is not a substrate for b-galactosidase, as the carbon-sulfur bond cannot be cleaved by the enzyme, so its concentration remains constant. The compound is widely used with X-Gal plates to screen for blue/white selection of recombinant bacteria.

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