Onar® EUB Eubacteria Detection Kit pro konvenční PCR (100 Testů)

Description

PCR kit for conventional, endpoint PCR of bacteria in various in situ biologicals including cell cultures and virus stocks.

Scope of Application

Applicable in research and industry for direct testing of cell cultures and biologicals. For QA application specific validation might be required. Intended for research use only. Not recommended for clinical diagnostics or testing of human samples.

Kit Components

Lyophilized Reaction Mix including the internal amplification control and polymerase

Reaction buffer

Lyophilized positive control DNA
PCR grade Water

Package Sizes

Primer/Nucleotides sets and are prepared in aliquots of 25 tests.

• Cat. No. 12-1025 25 Tests
  Cat. No. 12-1050 50 Tests
  Cat. No. 12-1100 100 Tests
  Cat. No. 12-1250 250 Tests

Result Evaluation

Gel electrophoresis

Required Consumables

PCR reaction tubes

Required Lab Devices

PCR cycler
Agarose gel electrophoresis and DNA stain
Pipettes and filter tips
Microcentrifuge

Storage

The kit should be stored at +2 to +8 °C. After rehydration the reagents must be stored at < -18 °C.

Fig. Amplified PCR products are visualized by standard gel electrophoresis.