PCRBIO HiFi Polymerase (200 UI)

• 50x higher fidelity than Taq DNA polymerase
• Increased PCR success rates with longer amplicons
• Advanced buffer chemistry including Mg and dNTPs
• High yields under standard and fast PCR conditions
• Efficient and specific amplification from complex templates including GC rich and AT rich sequences
• Stable at 25°C and 37°C for 4 weeks

The enhanced DNA binding of PCRBIO HiFi Polymerase allows for improved processivity, increasing yield and shortening cycling times (35 cycles of a 5kb amplicon in under 1.5hours). The enhanced efficiency of PCRBIO HiFi Polymerase minimises PCR inhibition, from impure samples such as colony PCR and direct PCR.

PCRBIO HiFi Polymerase uses the latest developments in DNA polymerase technology and buffer chemistry to enhance PCR speed, yield and specificity. The enzyme and buffer system are room temperature stable for 4 weeks and give superior PCR performance on complex templates such has mammalian genomic DNA. PCRBIO HiFi Polymerase performs consistently well on a broad range of templates including both GC and AT rich.

Applications

• High fidelity PCR
• Blunt-end cloning
• Site directed mutagenesis
• Next generation sequencing
• Crude sample PCR
• Colony PCR
• “Difficult” PCR - GC/AT rich DNA

Pack size: