Real-time PCR Internal Controls

Kit Internal DNA extraction control kits

Kit contents:
Lyophilised positive control primers/probe (150 reactions), positive control DNA or RNA template, RNAse/DNAse free water.

Probe fluorophore channel: FAM

Unique product features

• Perfect Internal control
• Taqman style probe
• Available to read through CY5, FAM, TAMRA, VIC or ROX channel.
• Primer limited to prevent interference with target gene primers

Negative test results are only valid when the suitability of the reagents, template and protocols have also been demonstrated. The Primerdesign real-time PCR internal control kit contains either control RNA or DNA. This can be spiked in to your sample prior to nucleic acid extraction or diluted and spiked directly in to your real-time PCR reaction. A positive signal of around Cq 25 indicates that your experimental procedure is working well.

The internal control kit can be multiplexed, or used in parallel with your test samples. The assay is primer limited and based on an obscure nucleic acid sequence so will not therefore interfere with the detection of your target sequence.