Precision DNase Kit

Unique product features

• Precise and efficient gDNA removal in 15 minutes
• No damage to RNA
• No addition of EDTA (PCR inhibitor)
• 100% recovery of all RNA

Precision DNAse is a novel product designed specifically for removing gDNA contamination from RNA prior to reverse transcription/real-time PCR. The Precision DNAse enzyme has an exceptional level of specificity towards dsDNA and no activity against RNA. In addition the kit's novel deactivation mechanism results in pure, undegraded RNA.

Unique 'stop' method protects RNA. Other DNase kits available are unsuitable for preparing RNA for real-time PCR. Many use an aggressive heat inactivation (>60C) which can result in Mg mediated RNA degradation. Others use a chemical stop solution containing EDTA (a known inhibitor of PCR). Whilst other kits offer a slurry of beads that capture the enzyme and allow centrifugal removal. The weakness with this final approach is that it makes recovering all of the RNA sample impossible. Our Precision DNAse kit is unique in having a heat labile enzyme. This means that enzyme activity is entirely destroyed by an 'RNA safe' 5 minute incubation at 55C.