Gradient system
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Use with 100 ml gradient mixer to make two 1 mm parallel denaturing gradient gels.
Compatible with microplates and thermal cycler blocks.
It is ideal for the study of mutations and DNA poly- morphisms critical in disease.
This system is primarily for parallel denaturing gradient electrophoresis. The full range of “DGGE” analysis can be performed in this unit.
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During Denaturing Gradient Gel Electrophoresis (DGGE) the buffer in the unit is heated using a 400- watt heater and the temperature controlled by an external temperature control connected to an in- ternal sensor. This allows the gel temperature to be set to the melting temperature of the amplified DNA polymorphism mutation of interest. The tempera- ture can be adjusted in 1°C increments up to 70°C, so the full range of DGGE analysis can be performed in this unit.
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The unit contains all the features of the successful E4300 maxi vertical unit and uses the same combs and accessories.
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Plate dimensions (WxL): 20x20 cm
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Gel dimensions (WxL): 16x17.5 cm
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Buffer volume: 1200...5600 ml
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Maximum sample capacity: 192 (48 per gel)
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Unit dimensions (WxDxH): 26x16x28 cm