AquaScreen® Escherichia coli

The AquaScreen® qPCR Detection kit series is specifically designed for the quantitative detection of common water pathogens such as Legionella, Pseudomonas, and E.coli.

The AquaScreen® approach includes sample preparation using  the AquaScreen® FastExtract kit and various qPCR detection kits. In contrast to time-consuming cell culture methods, the AquaScreen® approach needs less than six hours including sample preparation and qPCR to reliably detect water pathogens. In addition, our qPCR assay is superior in terms of sensitivity and specificity as the assay only detects the respective species.

The AquaScreen® qPCR assay is insensitive to contamination with other bacterial genera. Also, the assay’s robustness is unsurpassed with linear detection up to 3 x 106 particles per sample.

AquaScreen® Escherichia coli

Features

Type of PCR
Quantitative real-time PCR (qPCR)

Description
The AquaScreen® Escherichia coli qPCR Detection kit is used for DNA samples prepared with the AquaScreen® FastExtract kit for quantification of Escherichia coli in waters samples.

Scope of Application
Applicable in research and industry for QA testing of household and process water. Not recommended for clinical diagnostics, testing of human samples or pharmaceutical products.

Kit Components
Freeze-dried PCR mix with primer, probes, nucleotides, and polymerase
Rehydration Buffer
Freeze-dried Positive Control DNA
Freeze-dried Internal Amplification Control
PCR-grade Water

Package Sizes

Cat. No. 34-7025    25 reactions
Cat. No. 34-7100   100 reactions
Cat. No. 34-7250   250 reactions

Required Consumables
PCR reaction tubes
Optional: For quantification we recommend our E. coli PCR Quantification Standard (Cat. No. 52-0083).

Required Lab Equipment
qPCR cycler with filter sets for FAM™ and ROX™
Pipets and filter tips

Storage
The unopened kit should be stored at +2 to +8 °C. After rehydration the reagents must be stored below -18 °C.