AquaScreen® Legionella species

The AquaScreen® qPCR Detection kit series is specifically designed for the quantitative detection of common water pathogens such as Legionella, Pseudomonas, and E.coli.

The AquaScreen® approach includes sample preparation using  the AquaScreen® FastExtract kit and various qPCR detection kits. In contrast to time-consuming cell culture methods, the AquaScreen® approach needs less than six hours including sample preparation and qPCR to reliably detect water pathogens. In addition, our qPCR assay is superior in terms of sensitivity and specificity as the assay only detects the respective species.

The AquaScreen® qPCR assay is insensitive to contamination with other bacterial genera. Also, the assay’s robustness is unsurpassed with linear detection up to 3 x 106 particles per sample.

Features

Type of PCR
Quantitative real-time PCR (qPCR)

Description
The AquaScreen® Legionella species qPCR Detection Kit is used for DNA samples prepared with the AquaScreen® Fast Extract kit for quantification of Legionella in waters samples. The supplied primer set is specific for a broad range of legionella species, but does not detect other waterborne bacteria as required by ISO/TS 12869:2012.

Scope of Application
Applicable for water testing as described in ISO/TS 12869:2012, in research and industry for QA testing of process water. Not recommended for clinical diagnostics, testing of human samples or pharmaceutical products.

Kit Components
Freeze-dried PCR mix with primer, probes, nucleotides, and polymerase
Rehydration Buffer
Freeze-dried Positive Control DNA
Freeze-dried Internal Amplification Control
PCR-grade Water

Package Sizes
Cat. No. 33-2025    25 reactions
Cat. No. 33-2100   100 reactions
Cat. No. 33-2250   250 reactions

Required Consumables
PCR reaction tubes
Optional: For quantification we recommend our Legionella pneumophila PCR Quantification Standard (Cat. No. 52-0101).

Required Lab Equipment
qPCR cycler with filter sets for FAM™ and ROX™
Pipets and filter tips

Storage
The unopened kit should be stored at +2 to +8 °C. After rehydration the reagents must be stored below -18 °C.

Compliance
AFNOR XP T90-471 and ISO/TS 12869:2012