Midori Green Direct

MIDORIGreen Direct

MIDORIGreen Direct is a safe alternative to ethidium bromide for visualization of double-stranded DNA, single-stranded DNA, and RNA in agarose gels. In contrast to most other non-ethidium bromide based dyes, MIDORIGreen Direct is just added to your samples. The loading dye is already included.  You do not need to add any other loading dye to both gel matrix and running buffers

• Direct Staining of DNA/RNA
• No toxicity, non-carcinogenic
• Safe alternative of Ethidium Bromide
• Loading Dye is included
• Very low Background

Get the best signal to noise ratio

The direct staining of the DNA rather than the gel eliminates the background staining providing a perfect signal (Fig. 1). MIDORIGreenDirect was developed to work with Blue Light LED illuminators, but you can also use it with a regular UV transilluminator.

Fig.1: MIDORIGreen Direct detected by blue/green LED light vs ethidium bromide detected using UV-light.

Better Results for Downstream Application

The isolation of DNA from agarose gels enables downstream applications. It is well known that many dyes, such as ethidium bromide or even SYBR™ Green are strong enzyme inhibitors due to their intercalating properties. MIDORIGreen dyes bind to the DNA backbone. This results in a much higher efficiency for downstream applications, i.e. cloning (Fig. 2), seqeuncing, PCR, etc.

Fig.2: Better cloning efficiency with MIDORIGreen Direct. Increased number of transformed E. coli colonies with MIDORIGreen Direct than with ethidium bromide.

Download here our application note for more information

Proven Safety

MIDORIGreen Direct stain is non-carcinogenic and less mutagenic compared to ethidium bromide. Furthermore, we can state that MIDORIGreen Direct is impenetrable to latex gloves and cell membranes.

MIDORIGreen Direct is classified as non-hazardous to aquatic life, under CCR Title 22 regulation. Thus, small amounts of MIDORIGreenDirect stain can be safely released into the environment.

Download here the MIDORIGreen Direct safety report.

Loading Dye

MIDORIGreen Direct stains are provided in a form of 10X sample loading dyes and they are to be added to your samples only. You do not need to add any other dyes to the gel matrix nor to the running buffers.