Onar® Bacteria

Špecifikácia:

• Detekčný PCR kit s obsahom polymerázy a internej kontroly pre detekciu 45 bakteriálnych druhov v rôznych in-situ bunkových kultúrach.
• Vysoká citlivosť až 50 kópií na objem vzorky v priebehu 40 min.
• Detekcia menej ako 52 fg eubakteriálnej DNA, čo zodpovedá 12 genómom/ vzoriek.
• Dlhotrvajúca stabilita a jednoduché použitie len pomocou jednej štandardnej PCR

25 testov

PCR kit for conventional, endpoint PCR of bacteria in various in situ biologicals including cell cultures and virus stocks.

Scope of Application

Applicable in research and industry for direct testing of cell cultures and biologicals. For QA application specific validation might be required. Intended for research use only. Not recommended for clinical diagnostics or testing of human samples.

Kit Components

Lyophilized Reaction Mix including the internal amplification control and polymerase
Reaction buffer
Lyophilized positive control DNA
PCR grade Water

Package Sizes

Primer/Nucleotides sets and are prepared in aliquots of 25 tests.

• Cat. No. 12-1025      25  Tests
• Cat. No. 12-1050      50  Tests
• Cat. No. 12-1100    100  Tests
• Cat. No. 12-1250    250  Tests

Result Evaluation

Gel electrophoresis

Required Consumables

PCR reaction tubes

Required Lab Devices

PCR cycler
Agarose gel electrophoresis and DNA stain
Pipettes and filter tips
Microcentrifuge

Storage

The kit should be stored at +2 to +8 °C. After rehydration the reagents must be stored at < -18 °C.

Fig. Amplified PCR products are visualized by standard gel electrophoresis.