Venor®GeM Sample Preparation Kit

The Venor®GeM Sample Preparation Kit was optimized for the isolation and cleaning of genomic

mycoplasma DNA from cell culture materials or biopharmaceutical samples, including but not
restricted to raw materials, in process samples and final products, in combination with Minerva
Biolabs´ Venor®GeM Mycoplasma PCR Detection Kits.

Počet izolací: 50

37% of all cell culture supernatants, and almost all of confluent cell culture supernatants, inhibit PCR. Proficiency studies have demonstrated the importance of further DNA extraction for a sensitive PCR results even for unremarkable sample materials.

The Venor®GeM Sample Preparation Kit provides samples highly compatible with Minerva Biolabs Venor®GeM Mycoplasma PCR kits. A special protocol allows the purification of the samples in order to meet the high demands of the EP 2.6.7. The sample DNA is eluted in a volume of 50 µl in a high concentration and purity for maximum sensitivity of the PCR. The kit works with DNA extraction columns which are directly suitable for the treatment of up to 250 ul of your sample.

Scope of Application

For DNA extraction from:

Cell culture supernatants including cells and cell debris
ATMPs
Samples with polymers and glycerine (e.g. cryo conservation media)
Cell culture media
Protein solutions, incl. antibodies and enzymes.

Kit Components

Spin columns
Collection tubes
Conditioner, Wash and elution buffers

Required Consumables

Ethanol is required for the reconstitution of the wash buffer but not included in the kit. Microcentrifuge vials (1.5 ml) are needed to DNA elution.

At protein concentrations above 10 mg/ml in the sample pretreatment with Proteinase K is strongly recommended (Cat.-No. 11-9905).

Package Sizes

• Cat.-No. 56-1010      10 extractions
• Cat.-No. 56-1050      50 extractions
• Cat.-No. 56-1200    200 extractions

Storage

Store kit at +2 to +8°C. Shipment at ambient temperature.