qPCRBIO Probe 1-Step Virus Detect - pro ultra citlivou detekci virové RNA metodou 1-step RT-qPCR se sekvenčně specifickými sondami.
PCRBIO HS VeriFi™ Polymerase - is a versatile and robust proofreading enzyme with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.
PCRBIO VeriFi Polymerase - 100x higher fidelity than Taq DNA polymerase. Robust high fidelity enzyme engineered for all PCR applications where greater sequence accuracy is required, such as cloning, site-directed mutagenesis and sequencing
Mynox® Gold represents the further development of the classical Mynox®. Mynox® Gold is a combination of a standard antibiotic and the biological Mynox® reagent. One application comprises of a starter treatment that eliminates most of the mycoplasma particles without harming the cells. The main treatment kills all remaining mycoplasma.
No cytotoxicity – cytotoxicity is basically not detectable for most cell types. Most cell lines do not show any changes in morphology during treatment.
Low resistance risk – due to the combination of an antibiotic and a reagent showing a biophysical mode of action directly eliminating the mycoplasma, formation of resistances against Mynox® Gold is most unlikely.
Highly effective – the ratio for a permanent eradication is close to 100 %.
Mynox® Gold is intended for research use only. Not applicable for clinical treatment. Not applicable for the direct treatment of enveloped viruses or cells harboring Chlamydia or other bacteria as host systems. The antibiotic portion of the product might affect the integrated microorganisms.
1 application of Mynox® Gold contains 1 vial for the starter treatment and 3 vials for the main treatment. Each vial contains 500 µl as a sterile, ready-to-use solution.
1 application can be used to cure one cell culture permanently from mycoplasma burden.
Elimination success should be verified with a suitable mycoplasma detection system, e.g. Minerva Biolabs’s Venor®GeM Mycoplasma PCR Detection Kits.
6-well tissue culture plate or small tissue culture petri dish (6 mm Ø)
Regular cell culture plastics
Regular cell culture equipment (incubator, centrifuge, pipettes, etc.)
Kit components are maintainable at +2 to +8 °C for at least 6 months.
Fig. Cytotoxicity of Mynox® Gold on contaminated cells. Adherent cell lines (Vero, ML/S MDBK and RK13) were treated with Mynox®Gold according to the protocol 4 days of incubation. The cultures were inactivated and stained as described by Flick & Gifford. Untreated cultures had been used as reference (100 %).