PCRBIO HS VeriFi™ Polymerase - is a versatile and robust proofreading enzyme with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.
PCRBIO VeriFi Polymerase - 100x higher fidelity than Taq DNA polymerase. Robust high fidelity enzyme engineered for all PCR applications where greater sequence accuracy is required, such as cloning, site-directed mutagenesis and sequencing
The FastGene® Library Quantification kit is a quantitative
PCR based assay to determine the quantity of sequencing
competent NGS-libraries for the Illumina® sequencing
platform. The FastGene® Library Quantification kit comes
with primers, qPCR master mix, ROX additive and DNA
FastGene® Library Quantification kit can be used to
quantify Illumina® Libraries using a qPCR instrument.
For 500 Reactions
FastGene® NGS-Library Quantification Kit
The accurate quantification of next-generation sequencing (NGS) Illumina® Libraries is very important for maximizing data output and quality on every sequencing run. The FastGene® NGS-Library Quantification Kit contains all reagents for an accurate and reproducible qPCR-based quantification of Illumina® Libraries: The Kit comes with specific primers, qPCR Master Mix and DNA Standards.
Accurate and Sensitive qPCR-based Quantification
The FastGene® NGS Library Quantification kit was developed to reduce the variability in cluster density, resulting in improved sequencing results. The standards have been designed to allow a perfect concentration determination. The superior qPCR Master Mix included in the FastGene® NGS Library Quantification kit is able to detect even the smallest amount of libraries.
How did the NGS Library Quantification works?
The diluted library and the five known FastGene® NGS Standards are amplified using specific primers. The calibration curve is formed and used to determine the concentration of the sequencing competent libraries (Fig 1).
Fig. 1: Theoretical result of a library quantification (left) and calibration curve (right).
Specific detection of Libraries
FastGene® NGS Library Quantification kit comes with primers which are specific to the Illumina® adapters. Hence, only the libraries which have the adapter will be recognized by the FastGene® NGS Library Quantification kit.
FastGene® NGS Library Quantification kit comes with five very stable standards. These standards allow the formation of a standard curve to determine the concentration of a diluted library.
All components of the FastGene® NGS Library Quantification kit are submitted to repeated controls to ensure that there is no batch to batch difference. Additionally, freeze-thaw experiments of the components of the FastGene® NGS Library Quantification kit showed that no change was visible after repeatedly freeze-thawing the kit.