PCRBIO VeriFi Polymerase - 100x higher fidelity than Taq DNA polymerase. Robust high fidelity enzyme engineered for all PCR applications where greater sequence accuracy is required, such as cloning, site-directed mutagenesis and sequencing
MB Taq DNA Polymerase, 1 Unit/ul
Množství: 200 units
MB Taq DNA Polymerase, supplied in 10 mM Tris-HCl (pH 7.0), 50 mM KCl, 0.1 mM EDTA, and 50 % (v/v) glycerol. 10x reaction buffer.
100 mM MgCl2 solution.
If the polymerase is used in combination with Venor®GeM or Venor®GeM Advance the buffer provided with the polymerase is not required.
MB Taq DNA Polymerase is a highly processive 5´→3´ polymerase with no 3´→5´ exonuclease (proofreading) activity. At 72 °C and around pH 9 conditions, MB Taq DNA Polymerase reaches its highest level of activity, capable of amplifying a standard 1 kb strand of DNA in approximately 30 seconds.
The Taq‘s activity is blocked at ambient temperature, e.g. while
preparing your sample. The inhibition of the Taq polymerase is
completely reversed when the temperature is above 70 °C. Complete activation of the polymerase is achieved at 94 °C for 2 min. MB Taq DNA Polymerase is supplied with a 10x buffer for maximal yield and sensitivity which should fit most standard applications.