geNorm kits- reference gene selection

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  • FastGene® NGS-Library Quantification Kit -The accurate quantification of next-generation sequencing (NGS) Illumina® Libraries is very important for maximizing data output and quality on every sequencing run. The FastGene® NGS-Library Quantification Kit contains all reagents for an accurate and reproducible qPCR-based quantification of  Illumina® Libraries: The Kit comes with specific primers, qPCR Master Mix and DNA Standards.
  • PCRBIO HS VeriFi™ Polymerase - is a versatile and robust proofreading enzyme with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.

  • PCRBIO VeriFi Polymerase - 100x higher fidelity than Taq DNA polymerase. Robust high fidelity enzyme engineered for all PCR applications where greater sequence accuracy is required, such as cloning, site-directed mutagenesis and sequencing

  • Hledáme posilu do našeho týmu na místo ochodního zástupce - více informací zde




    •   NEXT GENERATION KITY pro izolaci nuklových kyselin od firmy BioECHO-nová technologie- významně méně kroků ve srovnání s protokoly "bind–wash–elute" (o 50-70% méně kroků) a tím drasticky zkrácený čas potřebný k izolaci (2-5xrychlejší, v závislosti na aplikaci)




    Double-dye (Hydrolysis) probe geNorm 12 gene kit

    Primer only geNorm 12 gene kit for use with SYBR green Zobrazit větší

    ge-DD-12-mo

    PrimerDesign

    Cena bez DPH 15 000 Kč

    Cena s DPH 18 150 Kč

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    Více informací

    For accurate gene quantification, it is essential to normalise real-time PCR data to a fixed reference; one that is not affected by your experimental conditions. There is increasing evidence that normalising to a single, randomly selected reference (house-keeping) gene e.g. Actin Beta, introduces large and variable errors into the analysis. geNorm is a system for selecting the best candidate reference gene for a given experimental scenario. To perform geNorm analysis the user measures the expression of 6 or 12 reference (house-keeping) genes in a representative set of their own samples. The geNorm software provided with the kit ranks the reference (house-keeping) genes in order of stability of expression. The output graphs and report simply identify the best reference (house-keeping) genes for that particular experiment.

    Each kit inlcudes 2 months free access (download instructions and activation code) to the geNorm analysis tool in qbase+, the world's leading real-time PCR data analysis software from Biogazelle.

    Kit contents
    Sufficient for 10 geNorm analyses: Lyophilised primers (and probe) for 6 or 12 reference (house-keeping) genes (200rxns each), RNAse/DNAse free water, access to the latest geNorm software in qbase+, 50% off discount code* for future reference (house-keeping) gene assay orders.

    How to download and install qBase+

    Species
    Kits are available for immediate delivery for human, mouse, rat, arabidopsis, c.elegans, xenopus topicalis, xenopus laevis, red fire ant, cucumber, tobacco, sheep, pig, cow and Zebra fish.  If you require a geNorm kit for another species please enquire.

      

    Mouse geNorm kit gene list

     

    Mus musculus actin, beta, cytoplasmic (ACTB), mRNA.
    Mus musculus glyceraldehyde-3-phosphate dehydrogenase (GAPDH), mRNA.
    Mus musculus ubiquitin C (UBC), mRNA.
    Mus musculus beta-2 microglobulin (B2M), mRNA.
    Mus musculus phospholipase A2 (YWHAZ), mRNA.
    Mus musculus ribosomal protein L13a (RPL13A), mRNA.
    Mus musculus calnexin (CANX), mRNA.
    Mus musculus cytochrome c-1 (CYC1), mRNA.
    Mus musculus succinate dehydrogenase complex, subunit A (SDHA), mRNA.
    Mus musculus 18S rRNA gene
    Mus musculus eukaryotic translation initiation factor 4A2 (EIF4A2), mRNA.
    Mus musculus ATP synthase subunit (ATP5B), mRNA.