PCRBIO HS VeriFi™ Polymerase - is a versatile and robust proofreading enzyme with AptaLock™ hot start technology for highly precise PCR. Improved DNA binding and increased processivity give significant improvements in speed, yield and sensitivity while also increasing PCR success rates of long and challenging templates.
PCRBIO VeriFi Polymerase - 100x higher fidelity than Taq DNA polymerase. Robust high fidelity enzyme engineered for all PCR applications where greater sequence accuracy is required, such as cloning, site-directed mutagenesis and sequencing
For accurate gene quantification, it is essential to normalise real-time PCR data to a fixed reference; one that is not affected by your experimental conditions. There is increasing evidence that normalising to a single, randomly selected reference (house-keeping) gene e.g. Actin Beta, introduces large and variable errors into the analysis. geNorm is a system for selecting the best candidate reference gene for a given experimental scenario. To perform geNorm analysis the user measures the expression of 6 or 12 reference (house-keeping) genes in a representative set of their own samples. The geNorm software provided with the kit ranks the reference (house-keeping) genes in order of stability of expression. The output graphs and report simply identify the best reference (house-keeping) genes for that particular experiment.
Each kit inlcudes 2 months free access (download instructions and activation code) to the geNorm analysis tool in qbase+, the world's leading real-time PCR data analysis software from Biogazelle.
Sufficient for 10 geNorm analyses: Lyophilised primers (and probe) for 6 or 12 reference (house-keeping) genes (200rxns each), RNAse/DNAse free water, access to the latest geNorm software in qbase+, 50% off discount code* for future reference (house-keeping) gene assay orders.
Kits are available for immediate delivery for human, mouse, rat, arabidopsis, c.elegans, xenopus topicalis, xenopus laevis, red fire ant, cucumber, tobacco, sheep, pig, cow and Zebra fish. If you require a geNorm kit for another species please enquire.
|Human geNorm kit gene list|
|Homo sapiens actin, beta (ACTB), mRNA.
Homo sapiens glyceraldehyde-3-phosphate dehydrogenase (GAPDH), mRNA.
Homo sapiens ubiquitin C (UBC), mRNA.
Homo sapiens beta-2-microglobulin (B2M), mRNA.
Homo sapiens phospholipase A2 (YWHAZ), mRNA.
Homo sapiens Ribosomal protein L13A (RPL13A), mRNA.
Homo sapiens 18S rRNA gene
Homo sapiens cytochrome c-1 (CYC1), mRNA.
Homo sapiens eukaryotic translation initiation factor 4A, isoform 2 (EIF4A2), mRNA.
Homo sapiens succinate dehydrogenase complex, (SDHA), mRNA.
Homo sapiens topoisomerase (DNA) I (TOP1), mRNA.
Homo sapiens ATP synthase, (ATP5B), mRNA